Figure 2.

Application of lumping on genome-scale metabolic models. The percentage of reactions with unknown ${\mathbf{\Delta }\mathit{G}}^0$ is in three GEMs, (a) iJR904, (b) iBsu1103, and (c) redHuman, reduced after lumping due to elimination of metabolites with unknown ${\mathit{\Delta }}_{\mathit{f}}{\mathit{G}}^0$. Shown is the percentage of unknown $\mathbf{\Delta }\mathit{G}$-values in relation to the total number of present metabolites (mets) and reactions, respectively, (rxns) before and after the lumping procedure was applied. The number of eliminated metabolites with unknown ${\mathit{\Delta }}_{\mathit{f}}{\mathit{G}}^0$ is with respect to the total number of metabolites. The investigated genome-scale models have low, high and medium number of unknown ${\mathbf{\Delta }\mathit{G}}^0$ and ${\mathit{\Delta }}_{\mathit{f}}{\mathit{G}}^0$, respectively, and pertain to organisms of increasing complexity.