Skip to main content
. 2021 Apr 7;12:656139. doi: 10.3389/fphys.2021.656139

FIGURE 3.

FIGURE 3

Senescent PASMCs promote PASMC proliferation by paracrine IL-6 and AX-024 of IL-6 inhibitor can inhibit cell proliferation by inhibiting paracrine IL-6 in hypoxia-treated PASMCs. (A) The paracrine level of IL-6 in the supernatant in PASMCs by ELISA kit. (B) The level of IL-6 in the culture medium of hypoxia-induced PASMC proliferation by different concentrations of AX-024. (C) The level of IL-6 in the culture medium in different groups in PASMCs after AX-024 treatment, Proliferation of PASMCs was measured by CCK8 assay after incubation with different AX-024 (0.01, 0.1, 1, 10, 100, 1,000 nmol/mL). (D) The cell viability detection in PASMCs by CCK8 after treating with different concentration of AX-024. (E) The protein expression of PCNA was measured by Western blot. (F) The mRNA expression of PCNA was measured by qPCR. Data are mean ± SEM, n = 3. ∗P < 0.05, ∗∗P < 0.01 vs. control, ∗∗∗P < 0.001 vs. control, #P < 0.05 vs. hypoxia, ##P < 0.01 vs. hypoxia; NS indicates no significance.