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. 2021 Apr 7;11:665379. doi: 10.3389/fcimb.2021.665379

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Copyright © 2021 Song, Zhang, Zhang, Chen, Lin, Han and Jiang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Purification and characterization of ZIKV NS5 MTase. (A) Purification of NS5 MTase and its mutants. SDS-PAGE (top) and Western blot (bottom) using anti-His-tag antibody analysis of the recombinant proteins. Lane M, protein marker. Lane 1, total cellular proteins containing induced recombinant WT-MTase. Lane 2, supernatant of cellular lysate containing induced recombinant WT-MTase. Lane 3, purified recombinant WT-MTase. Lane 4, purified recombinant D146A-MTase. Lane 5, purified recombinant K105A-MTase. (B) Activity of recombinant WT-MTase, D146A-MTase and K105A-MTase. Luminescence-based methyltransferase assay was used. The time course experiment was repeated three times. (C) Schematic for the principle of the luminescence-based methyltransferase assay.