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Surface plasmon resonance analysis for the binding of theaflavin to WT-MTase and its mutant. (A) Binding of theaflavin to WT-MTase protein. Various concentrations (0-200 μM) of theaflavin were injected into the chamber of a WT-MTase coated sensor chip. The change of response units (RU) is shown. (B) Binding of theaflavin to D146A-MTase protein.
