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. 2021 Apr 7;12:650582. doi: 10.3389/fpls.2021.650582

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Copyright © 2021 Yue, Wang, Yu, Chen, He, Li, Yu and Fan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biochemical characterization of recombinant HcBSMTs proteins. (A,B) Kinetic analysis for HcBSMT2 under different benzoic acid (BA) and salicylic acid (SA) concentrations. (C) Kinetic analysis for HcBSMT1 under different BA concentrations. The saturation curves were generated using Michaelis–Menten equation by hyperbolic regression. (D) Relative activity of HcBSMT1 with BA and SA. (E) Relative activity of HcBSMT2 with BA and SA. The activity of two enzymes with BA was set to 100%. Error bars indicate standard deviation of three independent measurements.