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. 2020 Dec 10;29(4):1439–1458. doi: 10.1016/j.ymthe.2020.12.009

Figure 1.

Figure 1

Microglia/macrophage activation at the boundary of the ischemic lesion increases over time after MCAo

(A) Schematic representation of the experimental protocol exploited to study microglial activation following MCAo. (B) Representative images of Iba1+ cells at the boundary of the ischemic lesion (0−500 μm) at days 1, 3, 7, and 14 after MCAo and in the corresponding region of the contralateral hemisphere at day 1 post-MCAo. Scale bars, 50 μm. Magnifications show ischemia-induced modifications of Iba1+ cell morphology; scale bars, 25 μm. (C) Representative images of CD16/32+ cells at the boundary of the ischemic lesion (0−500 μm) at days 1, 3, 7, and 14 after MCAo and in the corresponding region of the contralateral hemisphere at day 1 post-MCAo. Scale bars, 50 μm. (D) Representative images of Ym1+ cells at the boundary of the ischemic lesion (0−500 μm) at days 1, 3, 7, and 14 after MCAo and in the corresponding region of the contralateral hemisphere at day 1 post-MCAo. Scale bars, 50 μm. (E) Quantification of the density of Iba1+ cells at the boundary of the ischemic lesion (0−500 μm) and in the corresponding region of the contralateral hemisphere at days 1, 3, 7, and 14 after MCAo (n = 3). Data are expressed as mean ± SE. Two-way ANOVA (interaction p < 0.0001, time p < 0.0001, MCAo p < 0.0001) followed by Tukey’s post hoc analysis (p values relative to multiple comparisons are reported in the tables). (F) Quantification of the density of Iba1+ cells co-expressing the pro-inflammatory marker CD16/32 at the boundary of the ischemic lesion (0−500 μm) and in the corresponding region of the contralateral hemisphere at day 1, 3, 7, and 14 after MCAo (n = 3). Data are expressed as mean ± SE. Two-way ANOVA (interaction p < 0.0001, time p < 0.0001, MCAo p < 0.0001) followed by Tukey’s post hoc analysis (p values relative to multiple comparisons are reported in the tables). (G) Quantification of the density of Iba1+ cells co-expressing the pro-regenerative marker Ym1 at the boundary of the ischemic lesion (0−500 μm) and in the corresponding region of the contralateral hemisphere at days 1, 3, 7, and 14 after MCAo (n = 3). Data are expressed as mean ± SE. Two-way ANOVA (interaction p = 0.0967, time p = 0.2763, MCAo p < 0.0001) followed by Tukey’s post hoc analysis (p values relative to multiple comparisons are reported in the tables).