Figure 4.

DMOG broke the positive feedback loop of GDF7 expression

(A) The conditioned medium from P3 cells was collected and measured by ELISA. The GDF7 protein concentration was presented (n = 3 per group). (B) The methylation level of the GDF7 promoter and mRNA level of the GDF7 gene were measured by BSP and RT-PCR, respectively, in P3 cells (n = 3 per group). (C) The expression of pro-fibrotic genes, α-SMA and FN, and the level of N-cad were presented by immunofluorescence labeling in P3 cells (n = 3 per group). Scale bars, 20 μm. The data were represented as mean ± SD. Compared with NTM cells: ∗∗p < 0.01. Compared with P3 cells: #p < 0.05, ##p < 0.01. Compared with siRNA of GDF7 (siGDF7)-treated P3 cells: $$p < 0.01.