Figure 7.

GDF7 neutralization protected TM function in rhesus monkeys

(A) Simplified workflow of animal experiment. In brief, rhGDF7 was delivered into the anterior chamber three times on the first days of weeks 0, 2, and 4. The nGDF7 was injected into the anterior chamber for three times on the first days of weeks 10, 12, and 14. Ophthalmic examinations were conducted every 2 weeks throughout the entire process. The TM samples were harvested on the end of week 28. (B) The aqueous humor (AH) outflow facility was measured throughout the whole experiment. (C) Curves presented the IOP change in response to rhGDF7 delivery or/and nGDF7 treatment in monkey eyes (n = 4 per group). (D) Immunofluorescence labeling with N-cad, α-SMA, and FN was conducted in monkey TM tissues, and collagen accumulation was determined by Masson trichrome staining (n = 4 per group). Scale bars, 20 μm.