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. 2021 Apr 7;12:650522. doi: 10.3389/fimmu.2021.650522

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Copyright © 2021 Merino, Sablik, Korevaar, López-Iglesias, Ortiz-Virumbrales, Baan, Lombardo and Hoogduijn

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Membrane Particles on HUVEC apoptosis and adhesion molecules. HUVEC were stimulated with TNFα and treated with two concentration of MP (1:50,000, 1:100,000) and incubated at (A) 24h, and (B) 48h for the analysis of apoptosis. Surface expression of adhesion molecules on HUVEC was measured at the time point of 24h (C) ICAM-1, and (D) VEGFR2. Values are means ± SD of the mean fluorescent intensity of the receptors of 5 independent experiments each testing MP from 2 donors in each experiment.