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. 2021 Apr 7;12:650522. doi: 10.3389/fimmu.2021.650522

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Copyright © 2021 Merino, Sablik, Korevaar, López-Iglesias, Ortiz-Virumbrales, Baan, Lombardo and Hoogduijn

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of Membrane Particles on monocyte adhesion to TNFα-activated HUVEC. HUVEC were stimulated with TNFα and treated with MP at ratio 1:50,000. Subsequently, CFSE-labeled monocytes were added during 1h. (A) Representative fluorescent microscopy pictures show the adhered monocytes (white dots) to the HUVEC monolayer in non-stimulated and TNFα stimulated conditions. (B) Quantitative results of the monocyte adhesion assay analyzed by imageJ. No significance difference respect to the respective control (Non-Stimulated, and TNFα Stimulated HUVEC) was observed when MP were added *p < 0.05 compared to Non-Stimulated HUVEC.