Figure 4.

Influence of innate immune inhibitors on translation of sa-mRNA encoding LUC in BALB/c mice

(A) Time schedule of the animal experiment. (B–F) Wild-type BALB/c mice were electroporated intradermally with 1 μg of sa-mRNA encoding luciferase (LUC-sa-mRNA) in the presence of clobetasol propionate (B), hydrocortisone (C), dexamethasone (D), BAY11 (E), or ODN2088 (F). Topical treatment of the injection site with clobetasol propionate and hydrocortisone (25 μg/1 cm²) was performed 1 day prior to LUC-sa-mRNA injection and subsequently twice daily for 3 days. Dexamethasone was administered orally in drinking water for 4 days starting on day −1. BAY11 (25 μg) and ODN2088 (20 μg) were co-injected with the LUC-sa-mRNA (1 μg in 50 μl). LUC expression was determined by measuring the bioluminescence at the injection spot for 28 or 35 days. Each symbol represents the median of four individual mice, and the error bars indicate the interquartile range.