Figure 5.

The effect of cellulose purification and clobetasol on the type I IFN response and translation of sa-mRNAs

(A) dsRNA by-products in silica- and cellulose-purified sa-mRNAs (1,000 or 200 ng per dot) as analyzed by dot blotting with the J2 dsRNA-specific monoclonal antibody (mAb). (B) Type I IFN response kinetics after intradermal electroporation of silica- and cellulose-purified ZIKVac-sa-mRNA (1 μg) in IFN-β^+/Δβ-luc reporter mice with or without topical clobetasol treatment of the injection site. (C) The AUCs of the curves in (B) (n = 4). (D) LUC expression kinetics after intradermal electroporation of LUC-sa-mRNA in wild-type BABL/c mice after silica- or cellulose-based purification with or without clobetasol treatment of the injection site. (E) The AUCs of the curves in (D). Each symbol or bar represents the mean of four individual mice, and the error bars represent SEM. The statistical analysis of the data shown in (B) and (D) can be found in Table S1.