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. 2021 Jan 1;29(4):1512–1528. doi: 10.1016/j.ymthe.2020.12.034

Figure 7.

Figure 7

miR-1468-5p reprograms HDLECs via the HMBOX1-SOCS1-JAK2/STAT3 axis

(A) Western blot of phosphorylated and total JAK2/STA3 expression in HDLECs treated with miR-1468-5p mimics or indicated exosomes. (B and C) Relative luciferase activity of JAK2 (B) and STAT3 (C) pathway in HDLECs treated with miR-1468-5p mimics or indicated exosomes. (D) Western blot of indicated proteins in HDLECs with indicated treatment. (E and F) Schematic outlines of the predicted binding of HMBOX1 to the SOCS1 promoter (E) and the putative binding sites (F) in the promoter. (G) Relative luciferase activity of the SOCS1 promoter in HMBOX1 overexpression or knockdown HDLECs. (H) ChIP assays of the enrichment of HMBOX1 on indicated HMBOX1 binding sites (HBSs) in the promoter region of SOCS1 relative to IgG. The numeric values under the western blot bands represent the protein relative expression (the indicated protein/GAPDH). Error bars represent the mean ± SD of three independent experiments. ∗p < 0.05; ∗∗∗p < 0.001.