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. 2021 Jan 9;29(4):1411–1424. doi: 10.1016/j.ymthe.2021.01.005

Figure 4.

Figure 4

Rps4l regulates PASMC proliferation through its encoded peptide, RPS4XL

(A) MTT assay in hypoxic and control PASMCs transfected with lvRps4l, ORFmut, or lv-NC. (B) BrdU incorporation assay in hypoxic and control PASMCs transfected with lvRps4l, ORFmut, or lv-NC. (C) WB analysis of PCNA in hypoxic and control PASMCs transfected with lvRps4l, ORFmut, or lv-NC. (D) IF of Ki67 (scale bar, 10 μm) in hypoxic and control PASMCs transfected with lvRps4l, ORFmut, or lv-NC. (E) WB analysis of cyclin A, cyclin D, cyclin E, CDK1, CDK2, and CDK4 in hypoxic and control PASMCs transfected with lvRps4l, ORFmut, or lv-NC. (F) Scratch-wound assays in hypoxic and control PASMCs transfected with lvRps4l, ORFmut, or lv-NC. All values are represented as the mean ± SEM (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001; n ≥ 3).