TABLE 3.

Studies in which phosphoprotemic response to vasopressin was measured. Progress has been marked by a progressive improvement in sensitivity of phosphoproteomic methods used.

Year	Reference	Tissue	Number of Phosphopeptides Quantified	Quantification Method	Number of Phosphorylation Sites Changed	Number of Phosphorylation Sites Increased
2006	Hoffert et al., 2006	Rat IMCD suspensions	17	Label free	9	4
2010	Rinschen et al., 2010	Mouse mpkCCD cells	338	SILAC	45	18
2012	Hoffert et al., 2012	Rat IMCD suspensions	1427	Isobaric tags (iTRAQ)	44	30
2019	Deshpande et al., 2019	Rat IMCD suspensions	10,738	Isobaric tags (TMT)	219	156
2020	Datta et al., 2020	Mouse mpkCCD cells	19,221	SILAC	452	205

IMCD, inner medullary collecting duct; iTRAQ, isobaric tags for relative and absolute quantitation; mpkCCD, mouse epithelial cell culture line resembling cortical collecting duct; SILAC, stable isotope labeling in cell culture; TMT, tandem mass tag.