Fig. 4.

Extracellular miRNAs levels in sEVs secreted from L-OHP-resistant CRC cells. (A) The protein expression of sEVs secreted from SW620 cells was detected by Western blot analysis. CD9 and HSP70 were used as sEVs marker proteins. On the other hand, APOA1 and calnexin were used as non-sEVs marker proteins. (B, C) Amounts of miRNA in sEVs from CRC cells was detected using real-time RT-PCR and normalized by the amount of miR-16–5p. Each column represents the mean ± S.E.M. of three or four independent experiments (Student's t-test, *p < 0.05 significantly different from SW620 cells, Student–Newman–Keuls test, ^††p < 0.01 significantly different from HCT116 cells). ND: Not detected.