Figure 3.

AFM images of the full-length IRR in micelles at pH 7.0 (upper part, A–C) and pH 9.0 (lower part, D–F) in working buffer solution as described in Experimental procedures section with the U-shape of the ectodomain. White arrows point to the structure of tyrosine kinase domains (in A9, D12, D17). A and D, sets of images for the U-shape of the IRR. B and E, sequential scans (1–3 and 4–6) of the same full-length IRR molecule in micelle. C and F, histograms representing distributions of lengths of the ectodomain (L_E), heights of the ectodomain (H_E), heights of the tyrosine kinase domain (H_TK), and heights of micelles with the transmembrane part of the IRR (H_M). Scale bar is 20 nm, Z range (color-coded) is 4 nm (shown in image A1). AFM, atomic force microscopy; IRR, insulin receptor-related receptor.