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Differentiation medium II
| Reagent | Final concentration | Volume |
|---|---|---|
| DMEM/F12 | 44% (v/v) | 440 mL |
| FBS | 10% (v/v) | 50 mL |
| N2 supplement (100×) | 1% (v/v) | 5 mL |
| Penicillin-Streptomycin (100×) | 10,000 U/mL | 5 mL |
| 5 mM stock Retinoic acid | 0.5 μM | 50 μL |
| 0.1 M stock Taurine | 100 μM | 500 μL |
| Total | 500 mL |
