Fig. 3. Transgenic RORγt restores T cell development in RORγt^KO mice.

(A and B) Thymocyte development in RORγt^KO/Tg mice. Surface TCRβ abundance (A) and total numbers of thymocytes (B) were determined for WT, RORγt^KO, and RORγt^KO/Tg mice. Data in (A) are representative of three experiments. Data in (B) are means ± SEM of three independent experiments. (C) Quantitation of RORγt protein abundance in TCRβ^low preselection DP thymocytes from WT, RORγt^KO, and RORγt^KO/Tg mice. Data are means ± SEM of four independent experiments. (D) Surface γc abundance on TCRβ^low DP cells of WT, RORγt^KO, and RORγt^KO/Tg mice was assessed by flow cytometry. Left: Histograms are representative of four independent experiments. Right: Data are means ± SEM of four independent experiments with a total of five mice for each genotype.