Figure 3.
FTO is a direct target of SsD. (A) The molecular structure of SsD is shown. (B) Molecular docking illustrations showing the potential binding pocket of SsD in FTO. (C) Structural complex of FTO bound with SsD. The surrounding amino acids and α-KG are shown. (D) Western blots showing the effects of 1 µM SsD on the thermal stabilization of FTO protein. CETSA was assayed in cell lysates. The results were derived from three biological replicates. (E) NMR measurement of SsD interaction with FTO. (F) Determination of cellular uptake of SsD by LC-MS/MS quantitation. AML cells were treated with 1 µM SsD for 48 h. (G) In vitro quantification of inhibition by SsD on FTO demethylation activity of m6A in RNA using HPLC. (H) In a cell-free system, dot blot analysis of m6A abundance in the presence of various SsD concentrations and FTO protein is shown.