Figure 6.

SsD inhibits patient primary cells. (A) CCK-8 assays in the cells derived from two patients treated with SsD for 48 h. (B) Colony-forming assays of patient cells treated with SsD. Graphs showing the colony number from 3 independent experiments. Data are the mean ± SD; *P < 0.05, **P < 0.01. (C) Effect of SsD on cell-cycle arrest was determined using FACS based on PI staining in patient cells after 48 h of treatment. (D) After 48 h of SsD treatment, m⁶A abundance in the RNA samples was determined in primary patient cells using a dot blot assay. (E) TSQ traces of FTO demethylation of m⁶A in ssRNA in the absence and presence (1 µM SsD for 48 h) of SsD in patient cells. (F) qPCR expression analysis of the indicated genes in patients' SsD-treated cells. Data represent three independent experiments. (G) Western blot analysis of the indicated genes in patients' SsD-treated cells. (H) Leukemia-bearing mice were developed by intravenous injection of patient cells into 4-week old NSG mice. (I) WBC count of leukemia-bearing mice (n = 3) is shown. (J) Images show representative external views of the spleen with the histological analysis of H&E-stained spleen sections (Scale bars, 1 cm, middle), Wright-Giemsa-stained bone marrow (BM) cells (left), and external views of the lung with H&E-stained sections (right) from leukemia-bearing mice. (K-M) The quantification of blast cells (K), spleen weight (L), and tumor growing nodules on lung (M) are shown. (N) The survival curve of leukemia-bearing mice calculated by Kaplan-Meier survival analysis (n = 5) is shown.