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. 2021 Mar 31;11(12):5759–5777. doi: 10.7150/thno.57659

Figure 4.

Figure 4

HOXB5 promotes HCC metastasis through CXCL1/CXCR2 pathway-induced MDSCs infiltration. (A) MSDCs treated with conditioned media from Hepa1-6-HOXB5 cells with or without LV-shCXCL1 and SB265610 and migratory ability of MDSCs was shown. Bars represented the means ± SD of 3 independent experiments. (B-E) In vivo assays showed that CXCR2 inhibitor blocked the HOXB5-promoted HCC metastasis. (B) The indicated cells were implanted into the liver of C57BL/6 mice and bioluminescent images were shown. (C) Overall survival time of C56BL/6 mice was shown. (D) The number of lung metastatic nodules was counted. (E) HE staining showed metastatic nodules in the mice lung in different groups. (F) MDSCs and CD8+T cell were analyzed by flow cytometry. (G) IF showed the infiltration of MDSCs and CD8+T cell in different groups. (H-K) In vivo assay shown that anti-Gr-1 blocked the HOXB5-induced HCC metastasis. (H) The liver of C57BL/6 mice was implanted with cells and bioluminescent images in were shown. (I) Overall survival time of C56BL/6 mice in different groups was shown. (J) Lung metastatic nodules were counted. (K) HE staining shown metastatic nodules in the lung in different groups. (L) MDSCs and CD8+T cell were analyzed by flow cytometry. (M) The infiltration of MDSCs and CD8+T cell in different groups was shown by IF.