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. 2021 Mar 31;11(12):5813–5830. doi: 10.7150/thno.58731

Figure 4.

Figure 4

miR-146a-5p and miR-146a-5p suppress EMT, migration, and invasion of trophoblasts in vitro. (A-B) Expression levels of miR-146a-5p or miR-146b-5p and E-cadherin (E-cad), N-cadherin (N-cad), and vimentin (Vim) mRNA measured by RT-PCR in HTR-8 cells 48 h after transfection with miR-146a-5p or miR-146b-5p mimics or inhibitors, respectively. (C-D) Expression levels of E-cad, N-cad, and Vim protein measured by western blotting 48 h post transfection. (E-H) Migration and invasion capacities of HTR-8 cells treated with control or M1-EVs determined by wound healing and transwell assays, respectively. Representative images of migrated or invaded cells are shown (magnification, × 200). Error bars, SD. *P < 0.05, **P < 0.01, ***P < 0.001.