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. 2021 Mar 31;11(12):5813–5830. doi: 10.7150/thno.58731

Figure 5.

Figure 5

TRAF6 is the common target of miR-146a-5p and miR-146b-5p. (A) Four independent miRNA target databases were used to predict potential miRNAs. (B) Common target genes of miR-146a-5p and miR-146b-5p. (C) Schematic representation of the TRAF6 3'-UTR. Mutations were generated at the predicted miR-146a-5p and miR-146b-5p-binding sites. (D) Relative luciferase activity in HTR-8 cells cotransfected with MT-TRAF6 3'-UTR + miR-146a-5p control, MT-TRAF6 3'-UTR + miR-146a-5p mimic, WT-TRAF6 3'-UTR + inhibitor control, or WT-TRAF6 3'-UTR + miR-146a-5p inhibitor. (E) Relative luciferase activity in HTR-8 cells cotransfected with MT-TRAF6 3'-UTR + miR-146b-5p control, MT-TRAF6 3'-UTR + miR-146b-5p mimic, WT-TRAF6 3'-UTR + inhibitor control, or WT-TRAF6 3'-UTR + miR-146b-5p inhibitor. (F-H) Levels of TRAF6 mRNA and protein measured by RT-PCR and western blotting in HTR-8 cells 48 h after transfection with miR-146a-5p or miR-146b-5p mimics or inhibitors. (I-K) Levels of TRAF6 mRNA and protein measured by RT-PCR and western blotting in JEG3 cells 48 h after transfection with miR-146a-5p or miR-146b-5p mimics or inhibitors. Error bars, SD. *P < 0.05, **P < 0.01.