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. 2021 Mar 31;11(12):5813–5830. doi: 10.7150/thno.58731

Figure 6.

Figure 6

miR-146a-5p and miR-146b-5p suppress EMT, migration, and invasion of HTR-8 by downregulating TRAF6 expression. (A,B) Levels of TRAF6, E-cadherin (E-cad), N-cadherin (N-cad), and vimentin (Vim) mRNA and protein measured by RT-PCR and western blotting in HTR-8 cells 48 h after transfection with si-TRAF6 or Ad-TRAF6. (C,D) Migration and invasion capacities of HTR-8 cells transfected with si-TRAF6 or Ad-TRAF6 determined by wound healing and transwell assays, respectively. Representative images of migrated or invaded cells are shown (magnification, ×200). (E) Western blotting analysis of HTR-8 cells 72 h after transfection with miR-146a-5p mimic alone or in combination with miR-146b-5p mimic or Ad-TRAF6. (F,G) Migration and invasion capacities of HTR-8 cells determined by wound healing and transwell assays, respectively. Representative images of migrated or invaded cells are shown (magnification, × 200). Error bars, SD. *P < 0.05, **P < 0.01, *** P < 0.001.