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. 2021 Mar 31;11(12):5713–5727. doi: 10.7150/thno.55250

Figure 5.

Figure 5

Synergistic combination of CTX and DAS in human non-small cell lung cancer NCI-H1975 and NCI-H1299 cell lines. (A and B) In vitro cytotoxicity of CTX, DAS, free CTX/DAS mixture, and CD NAs against both cancer cells as determined by the CCK-8 assay. (C and D) Comparative analysis of CI values using the CTX and DAS combination. Molar ratios of CTX to DAS were 1:40 and 1:30 in H1975 and H1299 cells, respectively. (E and F) Fluorescence-activated cell sorting (FACS) analysis of NCI-H1975 cell apoptosis induced by different treatments for 48 h. (G and H) Dual AO/EB staining assay for analyzing cell death in NCI-H1975 cells with quantification of apoptotic cells by counting the EB-positive cells. Scale bars: 100 µm. (I) Long-term colony formation assay. The cells were grown in the presence of drugs and photographed after staining with a 0.1% crystal violet solution. (J and K) Microscopic observation of the proliferation of NCI-H1975 cells treated with different drugs using a Click-iT EdU assay with quantitative results. Scale bar: 100 µm. The data are presented as the means ± SD of three independent experiments. #p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.