Figure 4.

ZEB1 activates PFKM transcription through a non-canonic binding sequence. (A) Schematic diagram showing wildtype and truncated PFKM promoters. (B-C) Luciferase reporter vectors of wildtype PFKM promoter or its truncations were co-transfected with ZEB1 into HEK-293T cells, followed by determination of luciferase activity. The relative luciferase activity was normalized to cells transfected with wildtype PFKM promoter alone (column 1). (D-E) ChIP assay was performed in MHCC-97H cell line by using rabbit IgG and anti-ZEB1 antibody. CDH1 promoter serves as a positive control and ACTB promoter as a negative control (D). The data in (D) were quantified and normalized to input, and are presented as means±SD of three independent experiments (E) (***P<0.001, Student's t test).