Figure 2.

Knockdown of TUG1 Upregulated miR-200a-3p and Decreased TET2 and Inflammation. A: The expressions of TUG1 after TUG1 knockdown were measured. B: miR-200a-3p was detected by qRT-PCR under ORG 12 h/R 24 h condition. C: Cell viabilities were measured by CCK-8 assay in ORG 12 h/R 24 h -induced SH-SY5Y and SK-N-SH cells after transfection with shNC or shTUG1. D: ELISA measured IL-18 and IL-1β in ORG 12 h/R 24 h -induced cells with or without TUG1 knockdown. E: TET2 and inflammation-related proteins were detected by western blot in ORG 12 h/R 24 h -induced cells with or without TUG1 knockdown. Four independent experiments were repeated, with three replicates each time, *p < 0.05, **p < 0.01, ***p < 0.001.