Figure 6.

Demethylation of TUG1 by TET2 Promoted Inflammatory Response by Regulation of NLRP3. A: TUG1 expression was measured by qRT-PCR in ORG 12 h/R 24 h -injured cells after TET2 knockdown. B: The DNA methylation and expression of TUG1 were analyzed by methylation-specific PCR in ORG 12 h/R 24 h -induced SK-N-SH cells after TET2 knockdown. C: Cell viabilities were measured in ORG 12 h/R 24 h -induced SH-SY5Y and SK-N-SH cells after cotransfection with shTET2 and pcDNA3.1-TUG1. D: IL-18 and IL-1β were assessed by ELISA in ORG 12 h/R 24 h - injured cells after TET2 knockdown single or together with TUG1 overexpression. E: NLRP3, GSDMD-N, and Caspase-1 levels were assessed by western blot in ORG 12 h/R 24 h -induced injured cells after TET2 knockdown single or together with TUG1 overexpression. Four independent experiments were repeated, with three replicates each time, *p < 0.05, **p < 0.01, ***p < 0.001.