Figure 7.

Knockdown of TET2 Attenuated Ischemia/Reperfusion Injury by Downregulating TUG1 and Upregulating miR-200a-3p In Vivo. A: The neurologic deficit score of the MCAO mice treated with shNC or shTET2 was used to evaluate the neurologic behavior. B: The TTC staining images were used to assess the brain infarct area of the MCAO mice treated with shNC or shTET2. The relative infarct ratio was quantified to assess the effects of TET2 knockdown on brain damages induced by MCAO. C: The TUNEL staining was used to detect the levels of apoptotic cells in the MCAO mice treated with shNC or shTET2. The relative ratio of apoptotic cells was quantified in the MCAO mice treated with shNC or shTET2. D: The expression level of TUG1 and miR-200a-3p were detected using qRT-PCR in the MCAO mice treated with shNC or shTET2. E: The protein levels of TET2, NLRP3, Caspase-1, GADMDN, IL-18, and IL-1β were determined by western blot assay in the MCAO mice treated with shNC or shTET2. Three independent experiments were repeated, with five replicates each time, *p < 0.05, **p < 0.01, ***p < 0.001.