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The sequences of genes (F: forward primer R: reverse primer).
| Gene | primer | Primer sequences (5’→3′) |
|---|---|---|
| ALP | F | ACCACCACGAGAGTGAACCA |
| R | CGTTGTCTGAGTACCAGTCCC | |
| RUNX-2 | F | TGGTTACTGTCATGGCGGGTA |
| R | TCTCAGATCGTTGAACCTTGCTA | |
| OPN | F | ACGCCGACCAAGGAAAACTC |
| R | GTCCATAAACCACACTATCACCTCG | |
| β-actin | F | CATGTACGTTGCTATCCAGGC |
| R | CTCCTTAATGTCACGCACGAT |
