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. 2021 Apr 21;14:101. doi: 10.1186/s13068-021-01950-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Engineering the production of α-bisabolene in R. toruloides. a Correlation between P_GAPDH-BIS copy number and α-bisabolene titer at day 8 in SD medium containing 20 g/L glucose. b Production of α-bisabolene in SD medium containing 10 g/L glucose by three strains harboring the P_GAPDH-BIS cassette at various copy numbers (shown as points of corresponding color in A) and strain GB2, built by insertion of 6 copies of P_ANT-BIS into strain BIS3. BIS, α-bisabolene synthase from Abies grandis; P_GAPDH, R. toruloides glyceraldehyde 3-phosphate dehydrogenase promoter; P_ANT, R. toruloides adenine nucleotide translocase promoter