Fig. 2.

Production of α-bisabolene at 2-L scale. Strain GB2 was grown in medium containing DMR-EH supplemented with either 10 g/L yeast extract (bioreactor A6) or 5 g/L ammonium sulfate (bioreactors A7 and A8). Bioreactors A6 and A7 received a sodium hydroxide feed if the pH dropped below 5.0, while reactor A8 received no pH control. α-bisabolene titer (a) and pH (b) were monitored for 14 days. DMR-EH, Lignocellulosic hydrolysate prepared from corn stover by deacetylation and mechanical refining followed by enzymatic hydrolysis