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. 2021 Apr 21;14:101. doi: 10.1186/s13068-021-01950-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Engineering 1,8-cineole production in R. toruloides. Strains are described in Table 1. a Various combinations of HYP3 and AgGPPS2. b Different GPP synthases combined with P_ANT-HYP3. c Assorted strategies for expression of GgFPS(N144W) and HYP3, individually and as fusion proteins. Titers were measured following 5 days of cultivation in culture tubes containing YPD₁₀ medium. HYP3, 1,8-cineole synthase from Hypoxylon sp. E7406B; AgGPPS2, GPP synthase from A. grandis; P_ANT, R. toruloides adenine nucleotide translocase promoter; GgFPS, FPP synthase from Gallus gallus