Fig. 6.
Boosting production of 1,8-cineole in R. toruloides by overexpression of central mevalonate pathway enzymes, HMGR, MK, and PMK. a Comparison of three different methods for codon optimization (ECO, HC, and GO, described in Materials and Methods) for constructs harboring PANT-SpHMGR, PSKP1-McMK, and PDUF-ScPMK. b Various combinations of PANT-HMGR, PSKP1-MK, and PDUF-PMK, described in Table 1, all codon-optimized using the HC method. Strains were cultivated in flower plates containing GXY medium and 1,8-cineole was measured at 7 days. HMGR, 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase; MK, mevalonate kinase; PMK, phosphomevalonate kinase