Fig. 7.

Optimization of DMR-EH based media for the production of terpenes in R. toruloides. Strains were cultivated in flower plates and 1,8-cineole (a) and α-bisabolene (b) were quantified at 6 days and 12 days, respectively. YE, 10 g/L yeast extract; NP, 5 g/L ammonium sulfate and 100 mM potassium phosphate (pH 6.0); Sup, supplementation with 400 µg/L thiamine hydrochloride, 400 µg/L pyridoxine hydrochloride, 100 µg/L FeSO₄, and 1 mM Na₂SO₄. DMR-EH, Lignocellulosic hydrolysate prepared from corn stover by deacetylation and mechanical refining followed by enzymatic hydrolysis