Table 2.
List of full-length Fontainea venosa P450s identified in this study. Cellular location of the protein predicted using the TargetP program. ‘C’: chloroplast; ‘S’: secreted; ‘*’: unknown and ‘-’ not secreted
| Gene name | Length | Type | Family | Subfamily | Instability index (ii) | Clan | PI | Mol. Wt (kDa) | Loc |
|---|---|---|---|---|---|---|---|---|---|
| FvCYP97B1* | 391 | Non-A | CYP97 | CYP97B | 46.12 | CYP97 | 6.63 | 44.46 | – |
| FvCYP97B2* | 587 | Non-A | CYP97 | CYP97B | 43.71 | 8.04 | 66.16 | * | |
| FvCYP97A* | 632 | Non-A | CYP97 | CYP97A | 43.34 | 6.1 | 70.76 | * | |
| FvCYP97A1* | 419 | Non-A | CYP97 | CYP97A | 46.66 | 5.29 | 47.55 | * | |
| FvCYP97A2* | 441 | Non-A | CYP97 | CYP97A | 41.18 | 6.2 | 50.47 | * | |
| FvCYP97C1 | 566 | Non-A | CYP97 | CYP97C | 32.16 | 8.24 | 63.23 | * | |
| FvCYP704A1* | 520 | Non-A | CYP704 | CYP704A | 41.27 | CYP86 | 6.32 | 60.1 | S |
| FvCYP704A2* | 457 | Non-A | CYP704 | CYP704A | 41.94 | 5.43 | 53.17 | S | |
| FvCYP704A3* | 390 | Non-A | CYP704 | CYP704A | 41.62 | 8.48 | 44.12 | * | |
| FvCYP704A4* | 516 | Non-A | CYP704 | CYP704A | 39.07 | 8.18 | 59.67 | S | |
| FvCYP94D1* | 503 | Non-A | CYP94 | CYP94D | 38 | 7 | 58.19 | S | |
| FvCYP94A1 | 499 | Non-A | CYP94 | CYP94A | 40.16 | 8.18 | 56.37 | S | |
| FvCYP94C1* | 452 | Non-A | CYP94 | CYP94C | 47.09 | 9.42 | 51.82 | S | |
| FvCYP94B1* | 514 | Non-A | CYP94 | CYP94B | 42.88 | 8.95 | 59.52 | S | |
| FvCYP86A1 | 545 | Non-A | CYP86 | CYP86A | 45.31 | 8.65 | 62.02 | S | |
| FvCYP86B1* | 550 | Non-A | CYP86 | CYP86B | 41.66 | 8.74 | 63.41 | – | |
| FvCYP96A1 | 333 | Non-A | CYP96 | CYP96A | 49.51 | 8.26 | 39.24 | S | |
| FvCYP727B1 | 550 | Non-A | CYP72 | CYP727B | 33.13 | CYP727 | 8.7 | 62.33 | – |
| FvCYP727B2 | 548 | Non-A | CYP72 | CYP727B | 32.14 | 8.24 | 62.36 | – | |
| FvCYP714E1* | 382 | Non-A | CYP714 | CYP714E | 38.03 | CYP72 | 9.32 | 58.57 | S |
| FvCYP714E2* | 515 | Non-A | CYP714 | CYP714E | 35.26 | 9.2 | 43.72 | S | |
| FvCYP714A1 | 549 | Non-A | CYP714 | CYP714A | 39.3 | 9.28 | 62.67 | S | |
| FvCYP714A5* | 512 | Non-A | CYP714 | CYP714A | 36.34 | 9.36 | 58.8 | S | |
| FvCYP734A1 | 520 | Non-A | CYP734 | CYP734A | 45.3 | 8.49 | 59.34 | S | |
| FvCYP734A2 | 349 | Non-A | CYP734 | CYP734A | 46.25 | 6.02 | 39.75 | * | |
| FvCYP734A4* | 360 | Non-A | CYP734 | CYP734A | 38.02 | 8.74 | 40.24 | * | |
| FvCYP734A5* | 526 | Non-A | CYP734 | CYP734A | 41.86 | 9.59 | 60.09 | S | |
| FvCYP721A1 | 499 | Non-A | CYP721 | CYP721A | 43.06 | 9.14 | 57.1 | S | |
| FvCYP721A3 | 512 | Non-A | CYP721 | CYP721A | 42.85 | 9.14 | 58.1 | S | |
| FvCYP721A4 | 403 | Non-A | CYP721 | CYP721A | 45.12 | 9.17 | 46.32 | S | |
| FvCYP721A5 | 335 | Non-A | CYP721 | CYP721A | 42.39 | 8.48 | 38.76 | * | |
| FvCYP721A8* | 369 | Non-A | CYP72 | CYP721A | 48.81 | 9.61 | 42.9 | S | |
| FvCYP721A9* | 311 | Non-A | CYP72 | CYP721A | 51.09 | 7.7 | 36.23 | S | |
| FvCYP72D1* | 519 | Non-A | CYP72 | CYP72D | 32.21 | 8.02 | 59.24 | S | |
| FvCYP72D2* | 355 | Non-A | CYP72 | CYP72D | 38.3 | 5.53 | 40.77 | – | |
| FvCYP72A1 | 505 | Non-A | CYP72 | CYP72A | 45.33 | 9.21 | 58.87 | S | |
| FvCYP51G | 485 | Non-A | CYP51 | CYP51G | 36.63 | CYP51 | 7.72 | 55.74 | S |
| FvCYP710A1 | 411 | Non-A | CYP710 | CYP710A | 41.13 | CYP710 | 8.68 | 46.98 | * |
| FvCYP74A1 | 517 | Non-A | CYP74 | CYP74A | 48.45 | CYP74 | 8.07 | 57.45 | C |
| FvCYP74B1 | 496 | Non-A | CYP74 | CYP74B | 51.46 | 8.91 | 55.81 | C | |
| FvCYP74B2 | 492 | Non-A | CYP74 | CYP74B | 50.35 | 7.63 | 55.2 | C | |
| FvCYP707A1* | 347 | Non-A | CYP707 | CYP707A | 30.45 | CYP85 | 9.27 | 39.89 | S |
| FvCYP707A2 | 553 | Non-A | CYP707 | CYP707A | 43.04 | 9.23 | 63.52 | * | |
| FvCYP707A4 | 463 | Non-A | CYP707 | CYP707A | 40.45 | 9.05 | 52.43 | S | |
| FvCYP88A1 | 479 | Non-A | CYP88 | CYP88A | 40.52 | 9.09 | 54.67 | S | |
| FvCYP88A3 | 490 | Non-A | CYP88 | CYP88A | 41.57 | 9.05 | 56.76 | S | |
| FvCYP85A1 | 464 | Non-A | CYP85 | CYP85A | 41.83 | 9.23 | 53.3 | S | |
| FvCYP722A1* | 476 | Non-A | CYP722 | CYP722A | 41.48 | 9.13 | 54.67 | S | |
| FvCYP728D2 | 478 | Non-A | CYP728 | CYP728D | 41.32 | 9.46 | 54.05 | * | |
| FvCYP716E1 | 410 | Non-A | CYP716 | CYP716E | 38.77 | 8.92 | 47.87 | * | |
| FvCYP716C1* | 403 | Non-A | CYP716 | CYP716C | 44.65 | 8.92 | 46.21 | * | |
| FvCYP90A1* | 473 | Non-A | CYP90 | CYP90A | 41.04 | 9.18 | 54.75 | * | |
| FvCYP90D1 | 547 | Non-A | CYP90 | CYP90D | 47.54 | 9.55 | 63.9 | * | |
| FvCYP90D2 | 360 | Non-A | CYP90 | CYP90D | 40.74 | 9.09 | 41.2 | * | |
| FvCYP90B1 | 494 | Non-A | CYP90 | CYP90B | 47.54 | 8.86 | 56.73 | S | |
| FvCYP79D1* | 524 | A | CYP79 | CYP79D | 37.27 | CYP71 | 9.22 | 59.35 | S |
| FvCYP79D2* | 526 | A | CYP79 | CYP79D | 37.18 | 5.49 | 35.34 | S | |
| FvCYP93B1 | 512 | A | CYP93 | CYP93B | 30.31 | 8.61 | 58.18 | S | |
| FvCYP93D1 | 514 | A | CYP93 | CYP93D | 36.8 | 6.12 | 57.95 | S | |
| FvCYP93A1* | 515 | A | CYP93 | CYP93A | 35.37 | 6.39 | 58.08 | S | |
| FvCYP712C1 | 503 | A | CYP712 | CYP712C | 30.73 | 7.98 | 56.85 | S | |
| FvCYP712E1* | 349 | A | CYP712 | CYP712E | 42.4 | 5.58 | 40.66 | * | |
| FvCYP82C4* | 520 | A | CYP82 | CYP82C | 38.44 | 8.83 | 59.86 | S | |
| FvCYP82J2* | 360 | A | CYP82 | CYP82J | 34.04 | 9.29 | 40.5 | S | |
| FvCYP82J3* | 455 | A | CYP82 | CYP82J | 38.56 | 6.74 | 51.89 | – | |
| FvCYP82C5 | 319 | A | CYP82 | CYP82C | 21.55 | 5.95 | 35.37 | * | |
| FvCYP82D2* | 320 | A | CYP82 | CYP82D | 43.94 | 6.61 | 36.06 | * | |
| FvCYP82C7 | 514 | A | CYP82 | CYP82C | 49.83 | 7.61 | 58.84 | S | |
| FvCYP76D1 | 517 | A | CYP76 | CYP76D | 52.75 | 8.62 | 58.98 | S | |
| FvCYP81T1 | 307 | A | CYP81 | CYP81T | 45.32 | 9.52 | 35.76 | S | |
| FvCYP81T2 | 496 | A | CYP81 | CYP81T | 47.83 | 8.85 | 56.28 | S | |
| FvCYP81D1* | 501 | A | CYP81 | CYP81D | 47.78 | 8.9 | 57.91 | * | |
| FvCYP81D2* | 514 | A | CYP81 | CYP81D | 45.65 | 8.6 | 59.53 | S | |
| FvCYP81K1 | 505 | A | CYP81 | CYP81K | 44.71 | 8.22 | 57.46 | S | |
| FvCYP81K2 | 322 | A | CYP81 | CYP81K | 51.8 | 9.13 | 37.8 | S | |
| FvCYP76D2 | 498 | A | CYP76 | CYP76D | 48.92 | 8.39 | 56.22 | S | |
| FvCYP81S1 | 504 | A | CYP81 | CYP81S | 54.93 | 8.34 | 57.07 | S | |
| FvCYP81S2* | 306 | A | CYP81 | CYP81S | 52.02 | 9.05 | 35.19 | S | |
| FvCYP706C1 | 517 | A | CYP706 | CYP706C | 37.72 | 8.37 | 57.22 | S | |
| FvCYP76H1* | 503 | A | CYP76 | CYP76H | 38.56 | 8.69 | 57.06 | S | |
| FvCYP76I1* | 509 | A | CYP76 | CYP76I | 30.36 | 9.11 | 58.12 | S | |
| FvCYP76C2 | 495 | A | CYP76 | CYP76C | 41.51 | 6.88 | 56.79 | S | |
| FvCYP76D8* | 493 | A | CYP76 | CYP76D | 41.85 | 6.16 | 55 | S | |
| FvCYP76D7* | 494 | A | CYP76 | CYP76D | 37.17 | 6.19 | 55.01 | S | |
| FvCYP92A1 | 330 | A | CYP92 | CYP92A | 26.99 | 9.22 | 37.7 | * | |
| FvCYP84A2 | 499 | A | CYP84 | CYP84A | 45.27 | 6.07 | 56 | * | |
| FvCYP71B11* | 526 | A | CYP71 | CYP71B | 38.06 | 8.53 | 60.37 | S | |
| FvCYP71B2* | 342 | A | CYP71 | CYP71B | 45.86 | 8.64 | 39.28 | S | |
| FvCYP71* | 516 | A | CYP71 | 36.06 | 6.83 | 59.34 | * | ||
| FvCYP71AU1* | 311 | A | CYP71 | CYP71AU | 29.54 | 6.97 | 35.94 | S | |
| FvCYP71AU2* | 500 | A | CYP71 | CYP71AU | 32.65 | 6.15 | 56.48 | S | |
| FvCYP71A1 | 499 | A | CYP71 | CYP71A | 35.27 | 8.03 | 57.42 | * | |
| FvCYP83B1* | 498 | A | CYP83 | CYP83B | 42.49 | 9.07 | 57.05 | S | |
| FvCYP83B2* | 497 | A | CYP83 | CYP83B | 39.52 | 8.65 | 57.59 | S | |
| FvCYP83 | 339 | A | CYP83 | 34.22 | 9.91 | 38.67 | S | ||
| FvCYP71C1* | 506 | A | CYP71 | CYP71C | 36.92 | 7.65 | 57.95 | S | |
| FvCYP71C2* | 305 | A | CYP71 | CYP71C | 31.29 | 8.26 | 34.31 | S | |
| FvCYP71AP* | 511 | A | CYP71 | CYP71AP | 47.75 | 5.92 | 58.97 | S | |
| FvCYP71B12* | 301 | A | CYP71 | CYP71B | 43.28 | 9.69 | 27.62 | S | |
| FvCYP71B13* | 506 | A | CYP71 | CYP71B | 39.73 | 9.04 | 58.87 | S | |
| FvCYP71B6 | 349 | A | CYP71 | CYP71B | 47.68 | 9.56 | 40.18 | S | |
| FvCYP71D1 | 498 | A | CYP71 | CYP71D | 40 | 8.32 | 56.18 | S | |
| FvCYP71B7* | 514 | A | CYP71 | CYP71B | 43.43 | 8.73 | 58.25 | S | |
| FvCYP71B8* | 320 | A | CYP71 | CYP71B | 49.73 | 9.22 | 36.59 | S | |
| FvCYP71B9* | 343 | A | CYP71 | CYP71B | 37.29 | 6.28 | 38.62 | S | |
| FvCYP726A1 | 501 | A | CYP726 | CYP726A | 37.98 | 8.8 | 56.33 | * | |
| FvCYP71D2* | 503 | A | CYP71 | CYP71D | 43.37 | 7.71 | 56.75 | S | |
| FvCYP71B11* | 548 | A | CYP71 | CYP71B | 38.06 | 8.53 | 60.37 | * | |
| FvCYP98A1* | 508 | A | CYP98 | CYP98A | 39.14 | 7.77 | 57.96 | S | |
| FvCYP78A1* | 538 | A | CYP78 | CYP78A | 29.08 | 8.38 | 60.6 | S | |
| FvCYP78A2* | 468 | A | CYP78 | CYP78A | 39.04 | 8.93 | 52.54 | – | |
| FvCYP78A3 | 528 | A | CYP78 | CYP78A | 29.5 | 8.93 | 59.96 | * | |
| FvCYP78A4 | 514 | A | CYP78 | CYP78A | 37.91 | 9.16 | 58.96 | * | |
| FvCYP78A5 | 534 | A | CYP78 | CYP78A | 38.41 | 6.26 | 60.03 | S | |
| FvCYP73A1 | 505 | A | CYP73 | CYP73A | 45.67 | 9.11 | 58.38 | * | |
| FvCYP701A1 | 514 | A | CYP701 | CYP701A | 43.82 | 7.62 | 58.51 | S | |
| FvCYP701A2 | 408 | A | CYP701 | CYP701A | 45.2 | 6.15 | 46.67 | * | |
| FvCYP77B1* | 505 | A | CYP77 | CYP77B | 40 | 7.53 | 57.06 | * | |
| FvCYP77A1 | 502 | A | CYP77 | CYP77A | 37.73 | 9.29 | 57.89 | S | |
| FvCYP89A1 | 514 | A | CYP89 | CYP89A | 51.56 | 8.51 | 59.23 | S | |
| FvCYP89A2 | 338 | A | CYP89 | CYP89A | 49.41 | 5.64 | 39.59 | S | |
| FvCYP89A3 | 520 | A | CYP89 | CYP89A | 54.72 | 8.78 | 59.54 | * | |
| FvCYP89A4 | 517 | A | CYP89 | CYP89A | 49.02 | 8.77 | 59.14 | * |