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. 2021 Feb 24;296:100458. doi: 10.1016/j.jbc.2021.100458

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Equilibrium Ca²⁺titrations of a 2:1:1 complex of CaM+FGF12A+Na_V1.2_CTD.A, schematic depicting the possible binding of two CaM (CaM_N/blue, CaM_C/red) molecules the FGF12A (purple)+Na_V1.2_CTD (green) complex. The FGF12A LTP (green) and CaMBD (limon) are shown as cylinders. B, equilibrium Ca²⁺ titrations of CaM sites I and II in CaM+FGF12A+Na_V1.2_CTD (light blue, CaM:FGF12A:Na_V1.2_CTD = 2:1:1). The solid black and gray lines show reference titrations of CaM sites I and II in CaM+FGF12A_NTD and CaM+FGF12B+Na_V1.2_CTD, respectively. C, Equilibrium Ca²⁺ titrations of sites III and IV of CaM in CaM+FGF12A+Na_V1.2_CTD (hot pink, CaM:FGF12A:Na_V1.2_CTD = 2:1:1). The solid black and gray lines show reference titrations of CaM sites III and IV in CaM+FGF12A_NTD and CaM+FGF12B+Na_V1.2_CTD, respectively. D, maximum decrease in Phe fluorescence intensity in equilibrium Ca²⁺ titrations of CaM+FGF12B+Na_V1.2_CTD (blue), CaM+FGF12A+Na_V1.2_CTD (teal, CaM:FGF12A:Na_V1.2 = 1:1:1) and CaM+FGF12A+Na_V1.2_CTD (light blue, CaM:FGF12A:Na_V1.2_CTD = 2:1:1). E, maximum increase in Tyr fluorescence intensity in equilibrium Ca²⁺ titrations of CaM+FGF12B+Na_V1.2_CTD (red), CaM+FGF12A+Na_V1.2_CTD (pink, CaM:FGF12A:Na_V1.2 = 1:1:1), and CaM+FGF12A+Na_V1.2_CTD (hot pink, CaM:FGF12A:Na_V1.2_CTD = 2:1:1).