. 2021 Feb 24;296:100458. doi: 10.1016/j.jbc.2021.100458

Table 1.

FGF biosensor affinities for WT CaM

FGF_LTP	ΔG^a	K_d^b	N
FGF12A_LTP	−8.42 ± 0.24	576 nM	11

FGF_CaMBD	ΔG^a	K_d^b
FGF11A_CaMBD	−10.43 ± 0.18	19 nM	7
FGF12A_CaMBD	−9.40 ± 0.21	107 nM	9
FGF13A_CaMBD	−10.63 ± 0.02	13 nM	6
FGF14A_CaMBD	−9.87 ± 0.18	51 nM	7

ΔG in kcal/mol. Average and standard deviation based on N determinations of biosensors prepared from at least two independent cultures.

K_d, equilibrium dissociation constant, calculated from average value of ΔG reported in this table. Solution Conditions: 50 mM HEPES, 100 mM KCl, 50 μM EGTA, 5 mM NTA, 1 mM MgCl_2, 1.5 μM BSA, 500 μM DTT, 1 mM CaCl₂; pH 7.4, 22 °C.