Table 2.
FGF biosensor affinities for mutant CaM
| CaM1-148 | FGF12ACaMBD |
FGF13ACaMBD |
||||
|---|---|---|---|---|---|---|
| ΔGa | Kdb | N | ΔGa | Kdb | N | |
| E31Q/E67Q | −6.98 ± 0.07 | 6.74 μM | 3 | −7.82 ± 0.12 | 1.59 μM | 3 |
| E104Q/E140Q | −7.18 ± 0.04 | 4.73 μM | 4 | −7.18 ± 0.11 | 4.72 μM | 3 |
a
ΔG in kcal/mol. Average and standard deviation based on N titrations of biosensors prepared from at least two independent cultures.
b
Kd, equilibrium dissociation constant, calculated from average value of ΔG reported in this table. Solution Conditions: 50 mM HEPES, 100 mM KCl, 50 μM EGTA, 5 mM NTA, 1 mM MgCl2, 1.5 μM BSA, 500 μM DTT, 1 mM CaCl2; pH 7.4, 22 °C.