Table 3.

Effect of FGF12A_CaMBDp and FGF12A_NTD on Ca²⁺ binding of CaM

CaM1-148	Sites I and II			Sites III and IV
	ΔG1a (kcal/mol)	ΔG2a (kcal/mol)	Kd-appb (μM)	ΔG1a (kcal/mol)	ΔG2a (kcal/mol)	Kd-appb (μM)
-	−6.27 ± 0.21	−12.96 ± 0.09	15.92	−6.77 ± 0.09	−15.03 ± 0.04	2.73
+ FGF12ACaMBDp	−7.75 ± 0.07	−16.45 ± 0.04	0.81	−8.08 ± 0.14	−17.14 ± 0.05	0.45
+ FGF12ANTD	−7.69 ± 0.04	−16.48 ± 0.07	0.79	−8.27 ± 0.08	−16.89 ± 0.04	0.56

Solution conditions: 50 mM HEPES, 100 mM KCl, 1 mM MgCl₂, 50 μM EGTA, 5 mM NTA, 1 mM DTT, pH 7.4, 22 °C.

^aAverage ΔG values based on three independent determinations. Titrations fit to Equation 4.

^bK_d-app (apparent dissociation constant) was calculated from half of the average ΔG₂ value reported in this table.