. 2021 Feb 24;296:100458. doi: 10.1016/j.jbc.2021.100458

Table 5.

FGF biosensor sequences

FGF_LTP	Start Position^a	Sequence^b	End Position^a	pI^c
FGF12A_LTP	1	MAAAIASSLIRQKRQARESNSDR	23	11.54

FGF_CaMBD	Start Position^a	Sequence^b	End Position^a	pI^c
FGF11A_CaMBD	36	KSLCQKQLLILLSKVRLCGGRPARPDR	62	10.95
FGF12A_CaMBD	38	RSLCERHVLGVFSKVRFCSGRKRPVRR	64	11.88
FGF13A_CaMBD	35	KTSCDKNKLNVFSRVKLFGSKKRRRR	60	11.85
FGF14A_CaMBD	37	KTSCDKNKLNVFSRVKLFGSKKRRRR	63	11.85

Start and end positions are numbered according to the UniProt (https://www.uniprot.org/) convention (106).

FGF sequence inserted between the sequences for the YFP and CFP fluorophores in the FGF biosensor constructs (at KpnI and AgeI restriction sites).

Theoretical isoelectric point calculated with ExPASy (https://web.expasy.org/protparam/) (107).