Figure 2.
Quantification of viscosity, surface tension, and biomolecular diffusion in [RGRGG]5-dT40 condensates. (A) Variation of the condensate viscosity as a function of increasing [NaCl] is shown. The black points are the data and the red line is a fit using a stretched exponential function. The corresponding MSD plots are shown in Fig. S6. (B) Variation of the condensate surface tension as a function of increasing [NaCl] is shown. The black points are the data and the red line is a fit to a power law with exponent 3/2. The corresponding scaled relaxation times are shown in Fig. S7. The predicted critical value of [NaCl] is shown by a blue arrow. (C) Experimental determination of the critical [NaCl] for the same condensates as in (B) using the solution turbidity assay is shown. (D and E) Fluorescence autocorrelation curves are shown for the peptide and ssDNA chains at variable ionic strengths of the buffer, respectively (also see Fig. S5). (F) A plot summarizing FCS-derived diffusion coefficients of [RGRGG]5 and dT40 as a function of [NaCl] within the dense phase is given. All error bars represent ± 1 standard deviation.