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. Author manuscript; available in PMC: 2021 Jun 12.
Published in final edited form as: Biofabrication. 2020 Jun 12;12(3):035020. doi: 10.1088/1758-5090/ab906e

Figure 4.

Figure 4.

Effects of released small molecules on human monocyte-derived osteoclast morphology, size, TRAP activity, and gene expression. (A) TRAP staining images of osteoclasts treated with released media with RVS and SrRn (scale bars: 200 μm); (B) Measurement of osteoclast size based on TRAP staining images (at least three images were taken from each samples, and 5 samples were used for each scaffold group; ***p<0.001, n.s. indicates no significant difference); (C) TRAP activity (*p<0.05); (D) qPCR analysis of NFATc1, MMP-9, and CTSK genes for osteoclasts (n=3; *p<0.05, ***p < 0.001).