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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Microbes Infect. 2020 Jun 17;22(9):423–431. doi: 10.1016/j.micinf.2020.05.015

Figure 1.

Figure 1.

Kinetics of bacterial burden and host cell viability of naïve human MSCs infected with M. tuberculosis and M. bovis BCG. (A) Growth and survival of M. tuberculosis bacilli within naïve human BM-MSCs over a period of 22 days when infected at various MOIs. (B) The viability of M. tuberculosis-infected MSCs over a period of 22 days when infected at various MOIs. (C) Extracellular bacilli present in M. tuberculosis-infected naïve human BM-MSCs over a period of 22 days at various MOIs (D) Growth and survival of M. bovis BCG within naïve human BM-MSCs over a period of 12 days when infected at various MOIs. Open circles, MOI 1:1; open squares, MOI 1:5; open triangles, MOI 1:10; close circles, uninfected control. Bacterial burden was measured by CFU assay and cell viability was determined by Alamar Blue assay as described in Materials and Methods. Data are representative of 3 independent experiments (using MSCs from 3 different donors) carried out in duplicate and values are expressed as mean ± SD.