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Published in final edited form as: Microbes Infect. 2020 Jun 17;22(9):423–431. doi: 10.1016/j.micinf.2020.05.015

Figure 3. — Antibiotic susceptibility of intra-MSC M. tuberculosis. Dose-dependent killing kinetics of M. tuberculosis by first line antitubercular drugs Rifampin (RIF), isoniazid (INH), ethambutol (ETB) and pyrazinamide (PZA) when added alone at Day 0 (A), Day 7 (B), and Day 14 (C) post-infection. The effects of RIF, INH, ETB, and PZA at single doses of 2.5, 5, 10, and 20 μg/mL, and when added together is also shown (D). MSCs were infected at MOI 1:1. Various doses of drugs shown in legends are in microgram/well/10⁴ BM-MSCs. Culture medium and/or drugs were replaced every alternate day. Significant differences between treated samples and untreated controls were determined using 2-way ANOVA followed by Bonferroni’s post-hoc test (indicated with asterisks). Data are representative of 3 independent experiments (using MSCs from 3 different donors) carried out in duplicate. Bars and error bars represent means and SD, respectively.

Figure 3. — Antibiotic susceptibility of intra-MSC M. tuberculosis. Dose-dependent killing kinetics of M. tuberculosis by first line antitubercular drugs Rifampin (RIF), isoniazid (INH), ethambutol (ETB) and pyrazinamide (PZA) when added alone at Day 0 (A), Day 7 (B), and Day 14 (C) post-infection. The effects of RIF, INH, ETB, and PZA at single doses of 2.5, 5, 10, and 20 μg/mL, and when added together is also shown (D). MSCs were infected at MOI 1:1. Various doses of drugs shown in legends are in microgram/well/10⁴ BM-MSCs. Culture medium and/or drugs were replaced every alternate day. Significant differences between treated samples and untreated controls were determined using 2-way ANOVA followed by Bonferroni’s post-hoc test (indicated with asterisks). Data are representative of 3 independent experiments (using MSCs from 3 different donors) carried out in duplicate. Bars and error bars represent means and SD, respectively.