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. Author manuscript; available in PMC: 2021 Apr 21.
Published in final edited form as: Immunity. 2013 Sep 12;39(3):537–547. doi: 10.1016/j.immuni.2013.08.026

Figure 1. Enhanced Mycobacterial Uptake and Growth in Atg7–/– Macrophages.

Figure 1.

(A) Lysates of BMDMs from Atg7+/+ and Atg7−/− mice were evaluated by immunoblot analysis.(B) BMDMs were infected for 1 hr with BCG at an MOI of 10. Bacterial counts were estimated at 0, 3`, 5, and 7 days by the CFU assay.(C) BMDMs were infected with GFP-expressing H37Rv strain of Mtb (MOI 50) for 60 min. Phagocytic activity was measured by flow cytometry. Representative side scatter (SSC) versus GFP-Mtb dot plots with the corresponding mean fluorescence intensity (MFI) are shown. Mtb uptake was also measured by fluorescence microscopy at 1 hr after infection. DAPI was used to counterstain the nuclei. Scale bars represent 5 μm. Bacterial uptake was evaluated at the end of the 1 hr infection period by determining percent of infected cells (percentage of macrophages containing at least one bacterium). Representative images are shown.(D–F) BMDMs were infected with GFP-expressing Mtb (MOI 50). Bacterial uptake was quantified by fluorescence microscopy (D), flow cytometry (E), and CFU assay (F).

Data are mean ± SD, n = 3; *p < 0.05, **p < 0.001. See also Figure S1.