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. 2021 Apr 21;12:247. doi: 10.1186/s13287-021-02133-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Structural recapitulation of human lacrimal gland (LG) organoids. A Immunofluorescence staining for specific Markers, such as vimentin (VIM), E-cadherin (E-CAD), aquaporin5 (AQP5), alpha-smooth muscle actin (α-SMA), and lysozyme (LYZ), was performed in human LG tissue and organoid cultured in LG organoid medium (LGOM). B In the organoid, the proliferating cells were confirmed via BrdU assay. At 4 days after BrdU treatment, proliferating cell (BrdU and Ki76) and LG-specific (AQP5 and VIM) markers) were detected in organoids (Magnification; 100×, Scale bar; 50 µm). C TEM analysis was performed to confirm cellular ultrastructure in the organoid. Acinar cells (up; white dotted line) and secretory granules (down; red arrowhead) were detected in the LG organoid (Scale bar; 5 µm)