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. 2021 Apr 21;12:247. doi: 10.1186/s13287-021-02133-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Engraftment of lacrimal gland (LG) organoid into dry eye mouse model. A (a–b) Mouse lacrimal organoids were formed from lacrimal tissue of C57BL/6-GFP-Tg mice. The GFP signals were expressed in the formed organoid (Magnification; 10×, Scale bar; 500 µm) and A (c–d) their morphology was compared to that of mouse LG tissue through H&E staining (Magnification; 20×, Scale bar; 500 µm). B Inflammation-based dry eye disease (DED) animal model was established by concanavalin A (ConA) injection into the extra-orbital gland of the mouse. GFP-labeled organoids were transplanted into mouse lacrimal tissue after 7 days after ConA injection, and their engraftment was confirmed by GFP signal after 14 days after transplantation. Expression of aquaporin 5 (AQP5 ) was analyzed by immunofluorescence (Magnification; 40×, Scale bar; 100 µm)