Figure 4.

Detection limit of the DNA turbidity assay. (a) The ssDNA detection limit using Cas12a with the phase separation-based turbidity assay lies in the micromolar range. The tubes contained ∼0.86 μM activated Cas12a and 22 μM dT60 in 1× NEB 2.1 buffer and target concentrations as indicated. After 1 (top) or 3 h (bottom) of incubation at 37°C, pLL was added to a final concentration of 0.5 mg/mL. Note that these reaction conditions differ from those shown in the gel of Fig. 3a. (b) Turbidity measurements of reactions described in (a) are given. Error bars indicate SD of the mean from three independent repetitions. To see this figure in color, go online.